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Furthermore, the overexpression of idi genes of different origins in E. coli engineered for the production of lycopene has always led to carotenoid overproduction [147–149]; these findings fuel the debate about the non-essentiality and non-limiting role of the IDI reaction [150]. 40 J. Maury et al. 3 Metabolic Engineering of Microorganisms for Isoprenoid Production In the last decade there have been a number of investigations into the construction of engineered microorganisms with the ability to produce different isoprenoids.

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