By El Sayed H. El Ashry and Ahmed A. Kassem

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5 cups of peat moss with water, to this is added and mixed, 1 cup of vermiculite, & 1/4 to 1 cup of calcium carbonate. The casing is added to a depth of one inch. The aquarium is covered with a piece of polyethylene to keep moisture constant. Three or four times a day the I)olyethylene is removed and placed back to vent the nvironment. Stagnant air and excessive moisture will promote bacteria and mold overgrowth. The casing is occasionally misted to keep moist, but not soggy. Pin heads of mushrooms will form and the mushrooms will fruit.

The composition is moistened with 70 % alcohol and mill thoroughly. When the mixture is moistened too much the consistency is liquid to form tablets; the tablets come out looking like a blob of g dough. If the mixture is not moistened enough the tablets will crack fall apart. Trituration of Tablets The upper plate is placed on flat surface. The mixt (consistency of thick paste) is then pushed into the perforations on upper plate, the excess scrapped off, dusted with a fine powder sucrose and allowed to dry.

LSD-2S & TRYPTAMINE SYNTHESES Media byA. 2 Incubation at 24 degrees C. for 8 to 10 days produces 800 to 1,400 I1g ergotamine per mL from Claviceps purpurea. _ ... _---_ .. ---------------_ .... _-------------------_ .... --_. The following culture media uses mannitol as a carbon source. Mannitol maybe replaced by sucrose or glucose but should be sterilized by running through a Seitz filter. 2 with aqueous ammonia The following culture media for Claviceps purpurea was developed by Amici, Minghetti, Tonolo and Spalla (1964) at SOcieta Farmaceutici Italia.

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